Regulatory
Part:BBa_K553002:Design
Designed by: Giulio Bernardinelli Group: iGEM11_UNITS_Trieste (2011-09-17)
PTraI - TraR-OC8 HLA complex regulated promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the pcr amplification worked well, few mutation observed after sequencing that are present in our strain
PromTraFw
5' GTTTCTCGAATTCGCGGCCGCTTCTAGAGAAGTGCAGATTTGCACATGAAATCAACG 3'
PromTra rev""
5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATAGCGGAGAGAAAACATCTCATTCAGG 3'
pcr protocol:
95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞
Source
http://www.ncbi.nlm.nih.gov/nucleotide/8572673?report=genbank&log$=nuclalign&blast_rank=1&RID=795BNUZN014